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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
23/04/2021 |
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Data da última atualização: |
23/04/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
THÁ, E. L.; MATOS, M.; AVELINO, F.; LOMONACO, D.; RODRIGUES-SOUZA, I.; GAGOSIAN, V. S. C.; CESTARI, M. M.; MAGALHAES, W. L. E.; LEME, D. M. |
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Afiliação: |
EMANOELA LUNDGREN THÁ, Pós-graduanda da UFPR; MAILSON MATOS, Pós-graduando da UFPR; FRANCISCO AVELINO, Instituto Federal de Educação, Ciência e Tecnologia do Ceará; DIEGO LOMONACO, Universidade Federal do Ceará; ISISDORIS RODRIGUES-SOUZA, Pós-graduanda da UFPR; VIVIANA STEPHANIE COSTA GAGOSIAN, Pós-graduanda da UFPR; MARTA MARGARETE CESTARI, Pós-graduanda da UFPR; WASHINGTON LUIZ ESTEVES MAGALHAES, CNPF; DANIELA MORAIS LEME, UFPR. |
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Título: |
Safety aspects of kraft lignin fractions: discussions on the in chemico antioxidant activity and the induction of oxidative stress on a cell-based in vitro model. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
International Journal of Biological Macromolecules, v. 182, p. 977-986, Apr. 2021. |
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DOI: |
https://doi.org/10.1016/j.ijbiomac.2021.04.103 |
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Idioma: |
Inglês |
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Conteúdo: |
Lignin is a complex phenolic biopolymer present in plant cell walls and a by-product of the cellulose pulping industry. Lignin has functional properties, such as antioxidant activity, that make it a potential natural active ingredient for health-care products. However, not all safety aspects of lignin fractions have been adequately investigated. Herein, we evaluated the antioxidant and genotoxic potential of two hardwood kraft lignins (F3 and F5). The chemical characterization of F3 and F5 demonstrated their thermal stability and the presence of different phenolic units, while the DPPH assay confirmed the antioxidant activity of these lignin fractions. Despite being antioxidants in the DPPH assay, F3 and F5 were capable of generating intracellular reactive oxygen species (ROS) and subsequently causing oxidative DNA damage (Comet assay) in HepG2 cells. The biological relevance of the DPPH assay might be uncertain in some cases; therefore, we suggest combining in chemico tests with biological system-based tests to determine efficacy and safety levels of lignins and define appropriate applications of lignins for consumer products. Moreover, kraft lignins obtained by acid precipitation may pose risks to human health; however, as genotoxicity is not the sole endpoint of toxicity required in hazard assessments, additional toxicological evaluations are needed. |
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Palavras-Chave: |
DNA oxidation; DPPH assay; Hardwood lignin; Intracellular ROS; Lignina de foliosa; Oxidação do DNA. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02373naa a2200301 a 4500
001 2131487
005 2021-04-23
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.ijbiomac.2021.04.103$2DOI
100 1 $aTHÁ, E. L.
245 $aSafety aspects of kraft lignin fractions$bdiscussions on the in chemico antioxidant activity and the induction of oxidative stress on a cell-based in vitro model.$h[electronic resource]
260 $c2021
520 $aLignin is a complex phenolic biopolymer present in plant cell walls and a by-product of the cellulose pulping industry. Lignin has functional properties, such as antioxidant activity, that make it a potential natural active ingredient for health-care products. However, not all safety aspects of lignin fractions have been adequately investigated. Herein, we evaluated the antioxidant and genotoxic potential of two hardwood kraft lignins (F3 and F5). The chemical characterization of F3 and F5 demonstrated their thermal stability and the presence of different phenolic units, while the DPPH assay confirmed the antioxidant activity of these lignin fractions. Despite being antioxidants in the DPPH assay, F3 and F5 were capable of generating intracellular reactive oxygen species (ROS) and subsequently causing oxidative DNA damage (Comet assay) in HepG2 cells. The biological relevance of the DPPH assay might be uncertain in some cases; therefore, we suggest combining in chemico tests with biological system-based tests to determine efficacy and safety levels of lignins and define appropriate applications of lignins for consumer products. Moreover, kraft lignins obtained by acid precipitation may pose risks to human health; however, as genotoxicity is not the sole endpoint of toxicity required in hazard assessments, additional toxicological evaluations are needed.
653 $aDNA oxidation
653 $aDPPH assay
653 $aHardwood lignin
653 $aIntracellular ROS
653 $aLignina de foliosa
653 $aOxidação do DNA
700 1 $aMATOS, M.
700 1 $aAVELINO, F.
700 1 $aLOMONACO, D.
700 1 $aRODRIGUES-SOUZA, I.
700 1 $aGAGOSIAN, V. S. C.
700 1 $aCESTARI, M. M.
700 1 $aMAGALHAES, W. L. E.
700 1 $aLEME, D. M.
773 $tInternational Journal of Biological Macromolecules$gv. 182, p. 977-986, Apr. 2021.
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Embrapa Florestas (CNPF) |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
02/09/2005 |
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Data da última atualização: |
02/09/2005 |
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Autoria: |
BALDANI, J. I.; BALDANI, V. L. D. |
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Título: |
History on the biological nitrogen fixation research in graminaceous plants: special emphasis on the Brazilian experience. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Anais da Academia Brasileira de Ciências, Rio de Janeiro, v. 77, n. 3, p. 549-579, 2005. |
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Idioma: |
Inglês |
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Notas: |
História da pesquisa em fixação biológica de nitrogenio em gramíneas: enfase especial na experiência brasileira. |
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Conteúdo: |
This review covers the history on Biological Nitrogen Fixation (BNF) in Graminaceous plants grown in Brazil, and describes research progress made over the last 40 years, most of which was coordinated by Johanna Döbereiner. One notable accomplishment during this period was thw discovery of several nitrogen-fixing bacteria such as the rhizospheric (Beijerinckia fluminensis and Azotobacter paspali), associative (Azospirillum lipoferum, A. brasilense, A. amazonese) and the endophytic (Herbaspirillum seropedicae, H rubrisubalbicans, Gluconacetobacter diazotrophicus, Burlholderia brasilensis and B. tropica). The role of these diazotrophs in association with grasses, mainly cereal plants, has been studied and a lot of progress has been achieved in the ecological, physiological, biochemical, and genetic aspects. The mechanisms of colonization and infection of the plant tissues are better understood, and the BNF contribution to the soil/plant system has been determined. Inoculation studis with diazotrophs showed that endophytic bacetria have a much higher BNF contribution potential than associative diazotroph. In addition, it was found that the plant genotype influences the plant;bacteria association. Recent data suggest that more studies should be conducted on the endophytic association to strengthen the BNF potential. The ongoing genome sequencing programs: RIOGENE (Gluconacetobacter diazotrophicus) and GENOPAR (Herbaspirillum seropedicae) reflect the commitment to the BNF study in Brazil and should allow the country to continue in the forefront of research related to the BNF process in Graminaceous plants.
A presente revisão a história da Fixação Biológica de Nitrogênio (FBN) em Gramínea no Brasil, procurando mostrar a evolução da pesquisa na área iniciada a mais de 40 anos sob a liderança da pesquisadora Johanna Döbereiner. Um aspecto marcante deste período foi a descoberta de diversas bactérias fixadoras de nitrogênio atmosférico tais como as rizosfériacs (Beijerinckia fluminensis e Azotobacter paspali), e as endofíticas (Herbaspirillum seropedicae, H. rubrisubalbicans, Gluconacetobacter diazotrophicus, Burkholderia brasilensis e B. tropical). O papel destas bactérias diazotróficas em associação com as gramíneas, especialmente os cereais, tem sido estudado e muito se avançou sobre os aspecto ecológicos, fisiológicos, bioquímicos e genéticos. Os mecanismos de colonização e infecção dos tecidos das plantas foram melhor entendidos e a contribuição da FNB para sistema solo-planta foi determinado. Estudos de inoculação de cereais com bactérias diazotróficas, têm mostrado que as endofíticas têm o maior potencial de contribuição da FBN e que o genótipo da planta influencia na associação da planta/bactéria. Os avanços alcançados apontam para uma maior exploração e entendimento desta associação endofítica. Os programas de sequenciamento do genoma: RIOGENE (Gluconacetobacter diazotrophicus) e GENOPAR (Herbaspirillum seropedicae) mostram a importância da FBN no Brasil e devem permitir que o país continue na fronteira do conhecimento em relação ao processo de FBN em gramíneas. MenosThis review covers the history on Biological Nitrogen Fixation (BNF) in Graminaceous plants grown in Brazil, and describes research progress made over the last 40 years, most of which was coordinated by Johanna Döbereiner. One notable accomplishment during this period was thw discovery of several nitrogen-fixing bacteria such as the rhizospheric (Beijerinckia fluminensis and Azotobacter paspali), associative (Azospirillum lipoferum, A. brasilense, A. amazonese) and the endophytic (Herbaspirillum seropedicae, H rubrisubalbicans, Gluconacetobacter diazotrophicus, Burlholderia brasilensis and B. tropica). The role of these diazotrophs in association with grasses, mainly cereal plants, has been studied and a lot of progress has been achieved in the ecological, physiological, biochemical, and genetic aspects. The mechanisms of colonization and infection of the plant tissues are better understood, and the BNF contribution to the soil/plant system has been determined. Inoculation studis with diazotrophs showed that endophytic bacetria have a much higher BNF contribution potential than associative diazotroph. In addition, it was found that the plant genotype influences the plant;bacteria association. Recent data suggest that more studies should be conducted on the endophytic association to strengthen the BNF potential. The ongoing genome sequencing programs: RIOGENE (Gluconacetobacter diazotrophicus) and GENOPAR (Herbaspirillum seropedicae) reflect the commitment to the BNF study ... Mostrar Tudo |
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Palavras-Chave: |
Bactéria diazotrófica endofítica; Biological nitrogen fixation; Cereals; FBN; Fixação biológica de nitrogênio; Gramíneas; Inoculation. |
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Thesagro: |
Cereal; Inoculação. |
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Thesaurus NAL: |
grasses. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 04110naa a2200265 a 4500
001 1628407
005 2005-09-02
008 2005 bl --- 0-- u #d
100 1 $aBALDANI, J. I.
245 $aHistory on the biological nitrogen fixation research in graminaceous plants$bspecial emphasis on the Brazilian experience.
260 $c2005
500 $aHistória da pesquisa em fixação biológica de nitrogenio em gramíneas: enfase especial na experiência brasileira.
520 $aThis review covers the history on Biological Nitrogen Fixation (BNF) in Graminaceous plants grown in Brazil, and describes research progress made over the last 40 years, most of which was coordinated by Johanna Döbereiner. One notable accomplishment during this period was thw discovery of several nitrogen-fixing bacteria such as the rhizospheric (Beijerinckia fluminensis and Azotobacter paspali), associative (Azospirillum lipoferum, A. brasilense, A. amazonese) and the endophytic (Herbaspirillum seropedicae, H rubrisubalbicans, Gluconacetobacter diazotrophicus, Burlholderia brasilensis and B. tropica). The role of these diazotrophs in association with grasses, mainly cereal plants, has been studied and a lot of progress has been achieved in the ecological, physiological, biochemical, and genetic aspects. The mechanisms of colonization and infection of the plant tissues are better understood, and the BNF contribution to the soil/plant system has been determined. Inoculation studis with diazotrophs showed that endophytic bacetria have a much higher BNF contribution potential than associative diazotroph. In addition, it was found that the plant genotype influences the plant;bacteria association. Recent data suggest that more studies should be conducted on the endophytic association to strengthen the BNF potential. The ongoing genome sequencing programs: RIOGENE (Gluconacetobacter diazotrophicus) and GENOPAR (Herbaspirillum seropedicae) reflect the commitment to the BNF study in Brazil and should allow the country to continue in the forefront of research related to the BNF process in Graminaceous plants. A presente revisão a história da Fixação Biológica de Nitrogênio (FBN) em Gramínea no Brasil, procurando mostrar a evolução da pesquisa na área iniciada a mais de 40 anos sob a liderança da pesquisadora Johanna Döbereiner. Um aspecto marcante deste período foi a descoberta de diversas bactérias fixadoras de nitrogênio atmosférico tais como as rizosfériacs (Beijerinckia fluminensis e Azotobacter paspali), e as endofíticas (Herbaspirillum seropedicae, H. rubrisubalbicans, Gluconacetobacter diazotrophicus, Burkholderia brasilensis e B. tropical). O papel destas bactérias diazotróficas em associação com as gramíneas, especialmente os cereais, tem sido estudado e muito se avançou sobre os aspecto ecológicos, fisiológicos, bioquímicos e genéticos. Os mecanismos de colonização e infecção dos tecidos das plantas foram melhor entendidos e a contribuição da FNB para sistema solo-planta foi determinado. Estudos de inoculação de cereais com bactérias diazotróficas, têm mostrado que as endofíticas têm o maior potencial de contribuição da FBN e que o genótipo da planta influencia na associação da planta/bactéria. Os avanços alcançados apontam para uma maior exploração e entendimento desta associação endofítica. Os programas de sequenciamento do genoma: RIOGENE (Gluconacetobacter diazotrophicus) e GENOPAR (Herbaspirillum seropedicae) mostram a importância da FBN no Brasil e devem permitir que o país continue na fronteira do conhecimento em relação ao processo de FBN em gramíneas.
650 $agrasses
650 $aCereal
650 $aInoculação
653 $aBactéria diazotrófica endofítica
653 $aBiological nitrogen fixation
653 $aCereals
653 $aFBN
653 $aFixação biológica de nitrogênio
653 $aGramíneas
653 $aInoculation
700 1 $aBALDANI, V. L. D.
773 $tAnais da Academia Brasileira de Ciências, Rio de Janeiro$gv. 77, n. 3, p. 549-579, 2005.
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